Kittipong Laosuwan
University of Hong Kong, Hong Kong
Title: Antibacterial and anti-biofilm effect of Centella asiatica to periodontal pathogenic bacteria, Porphyromonas gingivalis
Biography
Biography: Kittipong Laosuwan
Abstract
Aim: The aims of this study are to investigate the abilities of Centella asiatica crude extract preventing the Porphyromonas gingivalis (P. gingivalis) biofilm formation and development and the ability to neutralize the virulent factors gingipain and fimbriae function.
Method: The P. gingivalis (strain ATCC33277) was cultured in suspension with TSB medium supplement with vitamin, hemin, menadione and yeast extract. The aqueous extraction was performed to crudely extract from Centella asiatica leaves with pure water, the crude extract was filtered and freeze dried before dissolved in PBS at concentration 0.125, 0.25, 0.5, 1, 2.5, 5, 10 mg/ml. The minimum inhibitory concentration (MIC) was determined for P. gingivalis in planktonic stage. The P. gingivalis biofilm was form in 96-wells plate with chemically defied medium for determining the biofilm formation inhibition effect and inhibitory effect on biofilm development by treating the bacteria before or during the biofilm formation respectively. The biofilm volume was measured with crystal violet assay. The toxicity to immortalized human periodontal ligament cell (ihPDL cell) was determined with MTT assay.
Result: The extract had no toxicity to ihPDL cell at all tested concentration (P>0.05). The extract was able to inhibit the P. gingivalis planktonic growth at concentration of 10 mg/ml. The biofilm volume was significantly reduced at concentration of 2.5 mg/ml for biofilm formation inhibition effect and at 0.25 mg/ml for inhibitory effect on biofilm development (P<0.05).
Conclusion: The Centella asiatica crude extract has a potential to inhibit biofilm formation and development even at lower concentration than MBC. This indicates that the extract might have alternative ways to inhibit the biofilm formation.