Arno Schintlmeister
Large-Instrument Facility for Advanced Isotope Research, University of Vienna
Biography
In collaboration with colleagues from the Department of Inorganic Chemistry and the
Vienna Research Platform for Translational Cancer Therapy Research, the technique’s
capability of multi-elemental, isotope selective topochemical analysis has been exploited for studying the sub-cellular uptake and distribution of isotopically labeled metal-based anticancer drugs within two- and three-dimensionally cultured malignant and non-malignant cells as well as within normal and tumor tissues. In this context, combining NanoSIMS with fluorescence microscopy and quantitative laser ablation inductively coupled mass spectrometry (LA-ICP-MS) for millimeter-scale chemical mapping and transmission electron microscopy (TEM) for single cell ultrastructure characterization has proven successful.
In collaboration with colleagues from the Department of Inorganic Chemistry and the
Vienna Research Platform for Translational Cancer Therapy Research, the technique’s
capability of multi-elemental, isotope selective topochemical analysis has been exploited for studying the sub-cellular uptake and distribution of isotopically labeled metal-based anticancer drugs within two- and three-dimensionally cultured malignant and non-malignant cells as well as within normal and tumor tissues. In this context, combining NanoSIMS with fluorescence microscopy and quantitative laser ablation inductively coupled mass spectrometry (LA-ICP-MS) for millimeter-scale chemical mapping and transmission electron microscopy (TEM) for single cell ultrastructure characterization has proven successful.
Abstract
Abstract : Nano-scale Secondary Ion Mass Spectrometry